Glutathione Peroxidase1 Gene Polymorphism (GPx1 Pro198Leu) in Association with Blighted Ovum

Abstract

Objective: To evaluate salivary GPx-1 gene polymorphism in pregnant
 women suffering from blighted ovum.
 Method: In this case-control study, 34 blighted ovum patients and
 34 healthy controls were studied. Genomic DNA was extracted from
 the saliva. The genotypes were determined by restriction fragment
 length polymorphism (RFLP-PCR) technique. Mad Calc (version
 12.1) was used for statistical analysis.
 Result: The frequency of CC, CT, and TT genotypes of GPx-1 gene
 were 41%, 44% and 14%, respectively in blighted ovum patients
 and in healthy volunteers were 44%, 47%, and 8.82-9%, respectively.
 After statistical analysis, the study showed no significant association
 between this polymorphism and blighted ovum (with p =
 0.63).
 Conclusion: These results indicated no significant association between
 GPx-1 (Pro198Leu) polymorphism and blighted ovum. However,
 further research is required to clarify the role of gene polymorphism
 in blighted ovum.
 [Indones J Obstet Gynecol 2016; 1: 15-18]
 Keywords: abortion, blighted ovum, glutathione peroxidase-1, GPx-
 1, RFLP-PCR