Glutathione Peroxidase-1 Pro198Leu Variant in Tuberculosis-infected Type2 Diabetes Mellitus Patients at Pulmonary Polyclinic Medan

Abstract

BACKGROUND: Glutathione peroxidase-1 (GPx-1) is an antioxidant that plays an important to the body protection system against oxidative stress. The GPx-1 polymorphism that has been identified in individual with several diseases. AIM: This study aimed to observe the distribution of GPx-1 Pro198Leu variant in tuberculosis (TB)-infected Type 2 diabetes mellitus (T2DM) patients at pulmonary polyclinic Medan. GPx-1 Pro198Leu polymorphism was genotyped in 40 T2DM patients that also infected by TB. MATERIALS AND METHODS: Analysis of GPx-1 Pro198Leu polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products were digested 4 h at 37°C with Apa1 restriction enzyme. The result of Apa1 enzyme digestion was visualized with 4% agarose. RESULTS: From 40 TB-infected T2DM patients, the frequency of genotypes CC,CT, and TT were, respectively, 82.5%, 17.5%, and 0%. The frequency of C allele was higher than T allele, i.e. 91.3% and 8.7%. CONCLUSION: It was concluded that in TB-infected T2DM patients at pulmonary polyclinic Medan, the GPx-1 Pro198Leu polymorphism has CC variant higher than CT, whereas the TT genotype was not found. The frequency of the C allele is higher than the T allele.