Glutathione mitigates meiotic defects in porcine oocytes exposed to beta-cypermethrin by regulating ROS levels

Abstract

Beta-cypermethrin (β-CYP) is a commonly used insecticide that is potentially toxic and has adverse effects on the health of both animals and humans. Studies have indicated that β-CYP damages organs like the liver, thyroid, intestinal tract, and uterus. However, the underlying mechanisms that β-CYP affects oocyte quality are poorly understood. According to our research, β-CYP exposure led to the aberrant assembly of spindles and alignment of chromosomes, resulting in porcine oocytes' defective nuclear maturation. Concurrently, β-CYP exposure perturbed the cytoplasmic maturation by disturbing the cortical granules (CGs), endoplasmic reticulum (ER), and mitochondrial integrity. It also led to accumulating reactive oxygen species (ROS) and apoptosis. We found that supplementation with glutathione (GSH) mitigated the meiotic defects induced by β-CYP exposure via regulating ROS levels. Our observations illustrate that β-CYP exposure adversely impacts oocyte meiotic maturation, and taking GSH supplementation is an effective strategy.