Glutathione-induced alleviation of cadmium toxicity in Zea mays

Abstract

Glutathione (GSH) is known to alleviate cadmium (Cd) stress in many plant species. However, the comprehensive mechanisms responsible for this effect in maize are still need more investigation. Here, a combination of physiological and molecular approaches was utilized in GSH-Cd treated maize seedlings, which revealed that GSH reversed the adverse effects of Cd, as reflected by plant growth, plant hormones, vacuole, stoma development, gene expression, etc. Plant growth, root cell viability, photosynthetic capacity, redox equilibrium, and cell ultrastructure recovery following GSH treatment, coupled with the strong up-regulation of Cd tolerance-related genes (e.g., phytochelatin synthetase-like protein, MYB and WRKY transcription factors, and CYP450), demonstrated the efficient activation of cellular defense against Cd toxicity. The addition of GSH significantly elevated GSH/GSSG ratio and the activity of γ-glutamylcysteine synthetase in both shoots and roots and markedly reduced Cd concentration in shoots. Ethylene emission rate and abscisic acid (ABA) content were significantly reduced after GSH application in the presence of Cd, except ABA content in leaves. These findings highlighted the significance of GSH in alleviating Cd-stress in maize and indicate a promising strategy for safe food production.