Abstract
Lung cancer remains one of the leading causes of cancer-related deaths worldwide, highlighting the urgent need for more effective and targeted therapies. In this study, we report the development of glutathione (GSH)-responsive, cysteamine-modified, nisin-functionalized gold nanoparticles (nisin-cyst-PE-GNPs) designed to enhance the cytotoxicity in lung cancer cells. Initially, PE-GNPs were synthesized using an extract from Penicillium melanoconidium (PE). These nanoparticles were subsequently functionalized with cysteamine-modified nisin (nisin-cyst) to target GSH-mediated anticancer activity. The resulting nisin-cyst-PE-GNPs were polydisperse, with an average particle size of 34.5 ± 3.32 nm. Characterization by XRD and FTIR confirmed the successful functionalization of nisin-cyst on the PE-GNPs. The nisin-cyst-PE-GNPs demonstrated both hemocompatibility and cytocompatibility at concentration below 10 μg/mL. The inhibitory concentration (IC50) values were 0.88 μg/mL for nisin-cyst-PE-GNPs, compared to 62.5 μg/mL for nisin and 75.01 μg/mL for PE-GNPs against the human lung cancer cell line (A549). AO/EB and DCFH-DA assays indicated that nisin-cyst-PE-GNPs significantly induced cell death in A549 cells. These findings suggest that nisin-cyst-PE-GNPs offer a promising nanotherapeutic approach for targeted lung cancer treatment.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
