Glutathione dependent enzyme activities in the foot of three freshwater mussel species in the Sava river, Serbia

Branka Perendija,Snezana Pajovic,Bojana Stojanovic,S.Z. Pavlovic,M.M. Paunovic,Zorica Saicic,P.D. Cakic,Tijana Kovacevic,Slavica Borkovic,R.M. Radojicic

doi:10.2298/abs0703169p

Branka Perendija, Snezana Pajovic + Show 8 more

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https://doi.org/10.2298/abs0703169p

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Journal: Arhiv za bioloske nauke	Publication Date: Jan 1, 2007
Citations: 9	License type: CC BY-NC-ND 4.0

Affiliation: Institute of Biophysics

Abstract

We investigated activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), and the phase II biotransformation enzyme glutathione-S-transferase (GST) in the foot of three freshwater mussel species: Unio pictorum (Up), Unio tumidus (Ut), and Sinanodonta woodiana (Sw) from the Sava River. Specific and total GSH-Px activity was lower in Sw than in Up and Ut. Total GR activity was higher in Up than in Sw. Specific GST activity was higher in Up than in Ut. Total GST activity was higher in Up than in Ut and Sw. Electrophoretic analysis of proteins shows species specifities between the investigated mussel species. Our study represents the first comprehensive report of the investigated glutathione-dependent enzyme activities in the foot of three freshwater mussel species from the Sava River, Serbia. .

Highlights

Activity of the antioxidant defense system can be increased or inhibited under chemical stress and antioxidant parameters represent biomarkers of interest (Doyotte et al, 1997)
Specific GST activity was significantly higher in U. pictorum than in U. tumidus (p
Total GST activity was considerably higher in U. pictorum than in U. tumidus and S. woodiana (p

Summary

Introduction

Activity of the antioxidant defense system can be increased or inhibited under chemical stress and antioxidant parameters represent biomarkers of interest (Doyotte et al, 1997). A number of antioxidant defense mechanisms are present in bivalve mollusks, including low-molecular-weight compounds and specially adapted enzymes (Winston, 1991). Use of the biotransformation enzymes involved in cellular detoxification as biomarkers of exposure to xenobiotics has been intensively studied in mollusks (P e - t e r s et al, 1999; Moreira et al, 2001). The first two enzymes are antioxidant defense enzymes, while GST is a phase II biotransformation enzyme. Glutathione exists in two forms: reduced glutathione (GSH) and oxidized glutathione (GSSG). The former is present usually in high concentrations in tissues and is one of the most important endogenous antioxidants. GSSG is toxic and can be used as an indication of oxidative stress (Parris and K i d d, 1997)

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