Abstract
Cadmium-sulfide crystallites form in the yeast Candida glabrata cultured in the presence of cadmium salts. The particles function to sequester and detoxify intracellular cadmium ions. The crystallites are peptide-coated, but the coating peptide varies with the nutrient conditions of the growth medium. When cultured in rich nutrient broth the yeast forms intracellular CdS particles coated with a mixture of glutathione and the gamma-glutamylcysteine dipeptide. In contrast, cultures in synthetic minimal medium yield particles coated with polymerized gamma EC peptides of general structure (gamma-Glu-Cys)n-Gly. Glutathione/gamma-glutamylcysteine particles exhibit properties analogous to quantum, semiconductor-type crystallites. The optical properties are dependent on particle size, and irradiation results in photoluminescence and photoreduction not observed in bulk CdS mineral. Aerobic irradiation leads to particle decomposition presumably via oxidation of the sulfide ions within the crystallite.
Highlights
Cadmium-sulfide crystallites form in the yeast Candida glabrata cultured in the presence of cadmium salts
C. glabruta cells cultured in the presence of cadmium sulfate
The natureof the coating peptideis dictated by the culturemedium
Summary
Cadmium-sulfide crystallites form in the yeast Candida glabrata cultured in the presence of cadmium salts. Purification andProperties of Metal-Glutathione Complexes-Exposure of C. glabratatocadmiumsaltsduring growth in a synthetic minimal medium yielded a single cadmium-sulfide complex containing yEC peptides. Cells grown in YTDmedium in thepresence of salts yielded ratio of GSH detectedin fraction 1 by the cyclic DTNB assay at least two cadmium-sulfide complexes resolved by ion ex- compared with Glu quantified by amino acid analysis was change and size permeation chromatography (Fig. 2 A ) .
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