Abstract
Two glutamine-requiring (Gln −) mutants of Bacillus subtilis SMY were deficient in glutamine synthetase activity in vitro . The Gln − mutants sporulated poorly unless glutamine was provided at high concentrations. The differential rate of histidase synthesis following induction was 4- to 6-fold higher in the Gln − mutants than in wild-type cells. In addition, glucose repression of utilization of alternative carbohydrates appeared to be partially relieved in the Gln − mutants.
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