Abstract
AbstractChemical derivatization of carbonyl compounds is a prerequisite for their determination in environmental and biological samples by liquid chromatography‐tandem mass spectrometry analysis. The main requirements regarding the good derivatization reagent are fast, selective, and quantitative reaction with carbonyl compounds and efficient fragmentation upon collision‐induced dissociation. We have prepared glutamic acid‐related hydrazine reagent and optimized derivatization procedure with benzaldehyde as a model carbonyl compound. The collision‐induced dissociation spectrum of the obtained hydrazone gave rich fragmentation pattern with numerous abundant fragment ions. Structure‐specific fragment ions were selected for the development of sensitive multiple reaction monitoring method.
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