Glucosyltransferase CsUGT78A14 Regulates Flavonols Accumulation and Reactive Oxygen Species Scavenging in Response to Cold Stress in Camellia sinensis.

Mingyue Zhao,Qiuyan Ban,Wilfried Schwab,Na Zhang,Tingting Jing,Jieyang Jin,Ting Gao,Jingming Wang,Chuankui Song

doi:10.3389/fpls.2019.01675

Abstract

Glycosyltransferases (UGTs) play diverse roles in cellular metabolism by altering regulatory metabolites activities. However, the physiological roles of most members of UGTs in crops in response to abiotic stresses are unknown. We have identified a novel glycosyltransferase CsUGT78A14 in tea crops, an important economic crops, whose expression is strongly induced by cold stress. Biochemical analyses confirmed that CsUGT78A14-1 showed the highest activity toward kaempferol and is involved in the biosynthesis of kaempferol-diglucoside, whereas the product of CsUGT78A14-2, which differs from CsUGT78A14-1 by a single amino acid, was identified as 3-O-glucoside. The accumulation of kaempferol monoglucosides and diglucosides was consistent with the expression levels of CsUGT78A14 in response to cold stress, as well as in different tissues and genotypes of tea plants. Down-regulation of CsUGT78A14 resulted in reduced accumulation of flavonols, reactive oxygen species (ROS) scavenging capacity and finally reduced tea plant stress tolerance under cold stress. The antioxidant capacity of flavonols aglycon was enhanced by glucosylation catalyzed by CsUGT78A14. The results demonstrate that CsUGT78A14 plays a critical role in cold stress by increasing flavonols accumulation and ROS scavenging capacity, providing novel insights into the biological role of UGTs and flavonoids in plants.

Highlights

Low temperatures, including chilling and/or freezing temperatures, are one of the most important environmental factors that adversely affect plant growth and agricultural productivity (Chinnusamy et al, 2007)
Multi-omics data deposited on the Tea Plant Information Archive (TPIA) showed that TEA007509 was strongly responsive to low temperatures (Li et al, 2019 and Wang et al, 2013)
The data indicated that Ala at 438 position of CsUGT78A14-1 plays a key role for the formation of the diglucoside in vitro and is located near the GSS motif, which was recently postulated as an important differentiation criterion between mono- and disaccharide-forming GTs (Huang et al, 2018; Figure S3)

Summary

Introduction

Low temperatures, including chilling and/or freezing temperatures, are one of the most important environmental factors that adversely affect plant growth and agricultural productivity (Chinnusamy et al, 2007). Plant growth and development can be severely effected by environmental stresses, including cold stress. Exposure of plants to low temperatures can cause an accumulation of reactive oxygen species (ROS) (Manthey et al, 2009), which are recognized as a common risk from abiotic stress and highly reactive and toxic, and affect many cellular functions (Gill and Tuteja, 2010). It is well known that the induction of the antioxidant capacity for ROS scavenging is important to protect plants in stresses, including cold stress (Ning et al, 2010; You et al, 2014; Hazman et al, 2015; Li et al, 2017). Flavonoids are important for plants to protect themselves under environmental stresses (Winkel-Shirley, 2002). The precise role(s) of glycosylation in abiotic stresses is still very difficult to understand until now

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Conclusion