Abstract
Glucosinolates (GLs) were characterized in the seed and root of Aurinia leucadea (Guss.) C. Koch and A. sinuata (L.) Griseb., and quantified according to the ISO 9167-1 official method based on the HPLC analysis of desulfo-GLs. Glucoalyssin (GAL, 1), glucobrassicanapin (GBN, 2) and glucoberteroin (GBE, 3) were the major GLs identified in A. leucadea and A. sinuata. GC/MS analysis of the volatile fractions obtained after enzyme hydrolysis showed that they mostly contain isothiocyanates (ITCs) originating from the parent GLs. On this basis and from previous reports, C-5 alkyl GLs 1, 2, and 3 can be considered as chemotaxonomic markers of the Aurinia genus.
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