Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage.

Mehede Hassan Rubel,Ill Sup Nou,Jong In Park,Hoy Taek Kim,Arif Hasan Khan Robin,Hee Jeong Jung,Md Abuyusuf,Ujjal Kumar Nath

doi:10.3390/plants9091121

Mehede Hassan Rubel, Ill Sup Nou + Show 6 more

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https://doi.org/10.3390/plants9091121

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Abstract

Cabbage (Brassica oleracea var. capitata) is an economically important crop in the family Brassicaceae. Black rot disease is a top ranked cabbage disease, which is caused by Xanthomonas campestris pv. campestris (Xcc) and may reduce 50% crop loss. Therefore, we need a clear understanding of black rot disease resistance for sustainable disease management. The secondary metabolites, like Glucosinolate (GSL) presents in Brassica species, which plays a potential role in the defense mechanism against pathogens. However, there is little known about GSL-regulated resistance mechanisms and GSL biosynthesis and the breakdown related gene expression after black rot disease infection in cabbage. In this study, relative expression of 43 biosynthetic and breakdown related GSLs were estimated in the black rot resistant and susceptible cabbage lines after Xcc inoculation. Ten different types of GSL from both aliphatic and indolic groups were identified in the contrasting cabbage lines by HPLC analysis, which included six aliphatic and four indolic compounds. In the resistant line, nine genes (MYB122-Bol026204, MYB34-Bol017062, AOP2-Bo9g006240, ST5c-Bol030757, CYP81F1-Bol017376, CYP81F2-Bol012237, CYP81F4-Bol032712, CYP81F4-Bol032714 and PEN2-Bol030092) showed consistent expression patterns. Pearson’s correlation coefficient showed positive and significant association between aliphatic GSL compounds and expression values of ST5c-Bol030757 and AOP2-Bo9g006240 genes as well as between indolic GSL compounds and the expression of MYB34-Bol017062, MYB122-Bol026204, CYP81F2-Bol012237, CYP81F4-Bol032712 and CYP81F4-Bol032714 genes. This study helps in understanding the role of GSL biosynthesis and breakdown related genes for resistance against black rot pathogen in cabbage, which could be further confirmed through functional characterization either by overexpression or knock-out mutation.

Highlights

The bacterial species Xanthomonas campestris infects a wide range of Brassica species, including cabbage
Two cabbage genotypes SCNU-C-4072 and SCNU-C-3383 were used as source of black rot disease resistance and susceptible lines, respectively, which were selected after screening of 59 inbred cabbage lines collected from the Department of Horticulture, Sunchon National University, Korea against
Fifty nine inbred cabbage lines were screened against black rot disease after infection of Xanthomonas campestris pv. campestris (Xcc) race 4

Summary

Introduction

The bacterial species Xanthomonas campestris infects a wide range of Brassica species, including cabbage. Black rot is a major disease in cabbage and was first identified in Korea in the 1970s [2]. It is a seed-borne disease and is caused by necrotrophic plant bacteria Xanthomonas campestris pv. This pathogen is distributed frequently around the world and causes 50% economic loss [4].

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