Abstract
Surface glucose transporters of intact muscles were photolabeled with the membrane impenneant ATB-[2- 3H]BMPA reagent and localized by autoradiography. We found sparse labeling of the glucose transporters by ATB-[2- 3H]BMPA on the sarcolemmal membrane around the muscle fiber. The majority of label was on the interior of the muscle fiber, at a discrete site which matched the distribution of AI junctions and which was presumed to be on the exterior surface of T-tubules. The amount of photolabel on the T-tubule was increased in response to insulin and wasblocked by cytochalasin B. These results support the concept that glucose transport may occur predominately across the T-tubule membrane under basal and insulin-stimulated conditions.
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