Abstract
Amperometric bioelectrodes constituted of carbon paste electrode incorporating poly(ethylene glycol)-modified glucose oxidase and mediators are studied. Influences of the nature of the mediators (anthraquinone, methylene blue, 2,6-dichloro-phenolindophenol, tetracyanoquinodimethane, 1,1'-dimethylferrocene) on the characteristics (potential window, apparent Michaelis constant, response time, stability) of the bioelectrodes are investigated. It is possible to show that the behaviour of the mediator is influenced by the pasting liquid contained in the carbon paste. Moreover, the water solubility of the mediator is the cause of a decrease of the apparent Michaelis constant in the case of methylene blue. A comparison between the stability of a low water soluble mediator (tetracyanoquinodimethane) and a more water soluble mediator (methylene blue) shows that the leakage of mediator from the carbon paste is not the cause of the lack of stability of such bioelectrodes. Among the various mediators tested, tetracyanoquinodimethane seems the most appropriate for the construction of a bioelectrode.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
