Abstract
A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponential model was used for analysing the fluorescence transients. Fractional intensities and mean lifetime were shown to be sensitive to the enzymatic reaction and were used for obtaining calibration curve for glucose concentration determination. The sensing system proposed achieved high resolution (up to 0.17 mM) glucose determination with a detection range from 0.4 mM to 5 mM.
Highlights
In the last years the employment of glucose oxidase (GOD) in glucose optical sensing has been largely investigated for clinical and industrial applications [1,2,3,4,5,6,7,8]
According to the results reported in Reference [31], the maximum of the fluorescence emission of free GOD was at 520 nm when the excitation is at 450 nm
We have presented and described a glucose sensing system that makes use of a monolithic silica gel matrix for entrapping glucose oxidase
Summary
In the last years the employment of glucose oxidase (GOD) in glucose optical sensing has been largely investigated for clinical and industrial applications [1,2,3,4,5,6,7,8]. It is well known that GOD is a homo-dimer composed of two identical 80-kDa subunits and two non-covalently bound flavin adenine dinucleotides complexes (FAD). The FAD structure is composed by flavoprotein and adenine dinucleotide (Figure 1). The isoalloxazine (ISO) ring is responsible for the light emission of FAD in the visible spectral range and is linked with adenine through hydrogen-bonding. Glucose Concentration Determination by Means of Fluorescence Emission Spectra of Soluble and Insoluble Glucose Oxidase: Some Useful Indications for Optical Fibre-Based Sensors.
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