Abstract
Glycolysis and the glucose phosphorylation capacity of livers from arthritic rats were studied because alterations in these parameters are suggested by some studies. Arthritis was induced in male albino rats (Wistar; 180-220 g). The animals were injected with 100 microl heat inactivated Mycobacterium tuberculosis suspended in mineral oil at a concentration of 0.5% (w/v). Animals showing lesions after 14 to 21 days were selected. Glucose phosphorylation was measured in the high speed supernatant fraction of liver homogenates and glycolysis in the isolated perfused liver. The glucose concentration for half-maximal rates was reduced from 18.32+/-5.69 in normal to 9.84+/-3.15 mM in arthritic rats (p = 0.024). Vmax was increased from 8.77+/-0.27 in normal to 11.49+/-0.29 nmol min(-1) mg protein(-1) in arthritic rats (p = 0.001). Perfused livers from arthritic rats showed a 2.43-fold higher rate of glycolysis. Livers from arthritic rats present a higher glucose phosphorylation capacity. Possibly this phenomenon is caused by circulating inflammatory mediators produced during adjuvant-induced arthritis.
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