Abstract
This paper describes a new technique of immobilization of enzyme on an amperometric biosensor to enhance response as well as stability. Glucose oxidase (GOD) was chosen as the model enzyme and cellulose acetate butyrate (CAB) as the polymer for encapsulation. The encapsulation was achieved by multiple emulsion technique. GOD was also immobilized by crosslinking with glutaradehyde directly on the surface of the electrode and results from a solution of 2 mM glucose were compared. The microencapsulated GOD electrodes proved to be superior to cross-linked GOD electrodes both with respect to response and stability.
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