Glucose biosensor on cellulose microfibers through layer-by-layer nanoassembly

Abstract

A paper-based glucose biosensor on cellulose wood microfiber has been developed based on the layer-by-layer (LbL) nanoassembly technique. LbL nanoassembly is a versatile and easy to use technique based on sequential deposition of oppositely charged polyelectrolytes or nanoparticles on surfaces of different shapes and sizes. It is emerging as a key nanomanufacturing approach, allowing the realization of novel nanometer-scale multi-layered materials and structures, designed to have desirable characteristics and properties (e.g. chemical, mechanical, and electrical). Using LbL nanoassembly, nanocomposites, consisting of conducting polymer poly (3,4-ethylenedioxythiophene) - poly(styrenesulfonate) (PEDOT-PSS) and glucose oxidase (GOx), have been coated to modify the surface of wood microfibers for paper-based glucose sensing application. Electrical characterization of the resulting nanocomposite-coated wood microfibers demonstrates good sensing response in the presence of glucose. Handsheets made of these microfibers have been realized for paper-based glucose biosensors. Unlike commercially available glucose biosensors that are fabricated on rigid substrates and use costly metal electrodes such as platinum, the paper-based glucose biosensors under development in this work are flexible, use polymer electrodes, and are intended to be cost effective.A paper-based glucose biosensor on cellulose wood microfiber has been developed based on the layer-by-layer (LbL) nanoassembly technique. LbL nanoassembly is a versatile and easy to use technique based on sequential deposition of oppositely charged polyelectrolytes or nanoparticles on surfaces of different shapes and sizes. It is emerging as a key nanomanufacturing approach, allowing the realization of novel nanometer-scale multi-layered materials and structures, designed to have desirable characteristics and properties (e.g. chemical, mechanical, and electrical). Using LbL nanoassembly, nanocomposites, consisting of conducting polymer poly (3,4-ethylenedioxythiophene) - poly(styrenesulfonate) (PEDOT-PSS) and glucose oxidase (GOx), have been coated to modify the surface of wood microfibers for paper-based glucose sensing application. Electrical characterization of the resulting nanocomposite-coated wood microfibers demonstrates good sensing response in the presence of glucose. Handsheets made of these mic...