Abstract
The “unprotected” Pt nanoclusters (average size 2nm) mixed with the nanoscale SiO2 particles (average size 13nm) were used as a glucose oxidase immobilization carrier to fabricate the amperometric glucose biosensor. The bioactivity of glucose oxidase (GOx) immobilized on the composite was maintained and the as-prepared biosensor demonstrated high sensitivity (3.85μAmM−1) and good stability in glucose solution. The Pt–SiO2 biosensor showed a detection limit of 1.5μM with a linear range from 0.27 to 4.08mM. In addition, the biosensor can be operated under wide pH range (pH 4.9–7.5) without great changes in its sensitivity. Cyclic voltammetry measurements showed a mixed controlled electrode reaction.
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