Glucose- and mannose-1,6-P 2 as activators of phosphofructokinase in red blood cells

Abstract

Glucose-1,6-P 2 and mannose-1,6-P 2 are concluded to be important activators from “reconstruction” experiments showing that the other known effectors of phosphofructokinase poise it at ∼ 0.1% of its V max, compared with the in vivo rate of ∼ 1%. These activators may explain the relative insensitivity of red cell glycolysis to fructose-1,6-P 2. Glucose-1,6-P 2 is elevated more than two-fold in pyruvate kinase deficient cells but not in cells from patients with alkalosis although both have increased levels of the inhibitor, glycerate-2,3-P 2.