Glucose- and H2O2-dual responsives drug delivery particle based on the boronic acid chemistry

Abstract

Herein, we report on responsive systems presenting dual response to two different stimuli, namely glucose and hydrogen peroxide which are interesting for biological applications. New chemical stimulus-responsive multilayer assemblies have been designed through the layer-by-layer deposition of oppositely charged biopolymer on either flat or spherical surfaces. (diethylaminoethyl-dextran hydrochloride/alginate derivative) polyelectrolytes pair was selected for hydrogen peroxide-response and (polylysine/alginate derivative) polyelectrolyte pair for glucose-response. Concentration-dependent glucose and hydrogen peroxide responsiveness were reached via the incorporation of phenylboronic acid moieties inside the alginate chain. Wettability and quartz crystal microbalance measurements showed that the alginate derivate self-assembles though electrostatically-driven interactions with the polycation. These techniques also evidenced that the multilayer assemblies present tuneable behaviour according to the addition of glucose- and/or- hydrogen. This approach was further extended to the preparation of smart microparticles using calcium carbonate as initial substrate. The porosity of these microparticles has been advantageously used to entrap a probe. Its release could then be triggered via glucose or hydrogen peroxide. The present system may be used to targeted drug delivery to diseased tissue or as sugar-sensing device for diabetic treatment.