Abstract
The concentration analyzer with high precision and wide range is the core device for monitoring the fermentation process. In this work, we designed and proposed a lowcost three-electrode glucose analyzer based on a self-made screen-printed enzyme biosensor chip, which has a Prussian blue (PB) nanocubic structure and leads to high sensitivity of 117.31 μAmM-1cm-2. The hardware design of the glucose analyzer can be divided into five critical parts, including digital, signal treatment system, power supply, motor-driven and the host computer. The signal treatment system is used to collect, convert and amplify the weak current signal generated by the biosensor. The digital circuit of the central processing unit of the analyzer is designed using the STM32F407ZET6. Besides, an external analog-to-digital converter is used to achieve high precision A/D conversion. The stability of the potentiostat is ensured by designing the precision power supply, hardware filtering, and algorithm filtering. The experimental results show that the glucose analyzer has a wide linear detection range from 1g/L to 120g/L and the coefficient of variation at 1g/L is 0.038, which exhibits excellent performance in stability and detection accuracy. The analyzer can be applied in the future for in-situ measurement of glucose concentration for its wide-range and high-precision detection capabilities.
Highlights
In order to obtain high efficiency with low cost, the continuous operation dominates the industrial operation in large-scale manufacturing, which strongly relies on the real-time control of the microbial reaction to realize an optimum transfer rate
A lowcost three-electrode glucose analyzer based on a selfmade screen-printed enzyme biosensor chip is developed, which includes the design of signal treatment system, digital circuit, precision power supply circuit, motor drive circuit and the host computer
As presented in Fig.6. 5 uL of PBS buffer was injected into the test cell to obtain ADV0 after turning o n the machine and activating the biosensor chip
Summary
In order to obtain high efficiency with low cost, the continuous operation dominates the industrial operation in large-scale manufacturing, which strongly relies on the real-time control of the microbial reaction to realize an optimum transfer rate. A lowcost three-electrode glucose analyzer based on a selfmade screen-printed enzyme biosensor chip is developed, which includes the design of signal treatment system, digital circuit, precision power supply circuit, motor drive circuit and the host computer. In previous work by our team [14,15,16], PB enzyme biosensor chips with nanocubic crystals were manufactured in batches by screen printing technology for the general detection of various physiological substances. The self-made enzyme biosensor chip has excellent selectivity, reproducibility and stability at a low operating potential of -0.05V
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