Abstract
 In breeding of winter rape (Brassica napus L. var. napus) using the OGU-INRA system, based on cytoplasmic male sterility (CMS), it is necessary to distinguish pollen-sterile lines, carrying CMS factors and the recessive fertility restorer alleles rfrf, from fertile lines, carrying at least one dominant restorer gene allele (Rfrf or RfRf). To grow plants till the flowering stage takes much time. The method was therefore modified using isozyme markers of glucose-6-phosphate isomerase (PGI) to distinguish male sterile (MS) from male-fertile lines in early stages. Since the restorer gene is tightly linked to the markers and the PGI isozymes can be distinguished by electrophoresis, the markers can be used to identify MS rape plants in early stages. Also, homozygous and heterozygous fertility-restored plants can be separated this way. In our work we tried to optimise the distinction of pollen-fertile and pollen-sterile rape plants with PGI isozyme markers, using vertical polyacrylamide gel electrophoresis (native-PAGE). The method will be used for the breeding of rapeseed with the OGU-INRA system.  
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
