Glucose-6-phosphate induced changed of stomatal aperture in an irradiance dependent manner

Abstract

Hexokinase catalyses hexose phosphorylation, which is the key step of sucrose metabolism. In this study, stomatal apertures of Arabidopsis epidermal peel were detected with or without exogenous application of mannose, fructose, glucose, and glucose-6-phosphate (G-6P). The results here showed that G-6P, but not glucose itself, induces stomatal closure in Arabidopsis. Furthermore, detection of stomatal apertures of Arabidopsis hexokinase loss of function with exogenous application of glucose showed that glucose induced stomatal closure was not due to osmotic pressure and it triggered guard cell ROS production depend on hexokinase activity. The effect of irradiance and G-6P on regulation of Arabidopsis stomatal aperture was investigated. The data obtained here indicated that G-6P induced changes of stomatal aperture depend on irradiance.