Glucose-6-phosphate dehydrogenase enzyme stability in filter paper dried blood spots

Abstract

ObjectivePrior to initial distribution of Glucose-6-phosphate dehydrogenase (G6PD) proficiency testing (PT) materials, we evaluated G6PD enzyme stability in dried blood spots (DBS) under various temperature and humidity environments to develop storage and usage guidelines for our new materials. Design & methodsWe prepared fresh G6PD-normal DBS materials and conducted stability evaluations of daily use and short and long-term storage under various temperature and humidity environments. ResultsG6PD DBS PT materials retained 92% of initial activity after 30days of use at 4°C. Materials stored at −20°C and 4°C with desiccant for 30days retained 95% and 90% of initial activity, respectively. When stored for one year at −20°C or six months at 4°C specimens retained >90% of initial activity. Specimens stored at 37°C with desiccant lost 10% activity in three days. At the end of 30days, specimens stored under ‘Extreme’—humidity >50% without desiccant— conditions at 37°C assayed below the NSQAP cut off for G6PD. Humidity exacerbated loss of enzyme activity with increasing temperature and time duration. ConclusionData suggest that G6PD PT materials can be stored at 4°C and used for up to one month and can be stored at −20°C for one year and yield >90% enzyme activity. Exposure to warm temperatures, especially with elevated humidity, should be avoided. Desiccant should always be used to mitigate humidity effects.