Abstract
Glucose 1,6-diphosphate concentrations in 10 mouse tissues were found to range from 70 to 80 µmoles per kg in brain and red blood cells to 7 to 10 µmoles per kg in testes and pancreas. There was no discernible correlation between levels of glucose 1,6-diphosphate and levels of glucose 6-phosphate, uridine diphosphoglucose or phosphoglucomutase, or Michaelis constants of phosphoglucomutase for its coenzyme. Ischemia caused a rapid decrease in glucose 1,6-diphosphate level in brain, whereas there was little or no effect of starvation on the level in liver, or of maximal electrical stimulation on the level in muscle. Evidence is presented to show that glucose 1,6-di-P in muscle can be synthesized by P-glucomutase itself from fructose-1,6-di-P and glucose-6-P (or glucose-1-P). The rate of synthesis is only about 0.015% of the mutase rate for glucose-1-P, but this would be sufficient to synthesize the glucose-1,6-di-P in mouse muscle in 3 min. The equilibrium constant is about 12 for (fructose-1,6-di-P) (glucose-6-P):(fructose-6-P) (glucose-1,6-di-P).
Highlights
Tissueswere found to range from 70 to 80 pmolesper kg in can by synthesized by P-glucomutasefrom fructose-l, 6-di-P brain and red blood cells to 7 to 10 pmoles per kg in testes and glucose-6-P
Evidence is presented to show that glucose 1,6-di-P in concerningthe reaction of fructose phosphateswith P-glucomumuscle can be synthesized by P-glucomutase itself from tase[5]
Tissue Levels of Glucose-1, B-&P-The results presented raise a question as to whether the tissue levels of glucose-1,6-di-P
Summary
Di-P were examinedto seewhy this substanceis so labile in z&o, and what processmay normally maintain the tissuelevel. Glucose 1,6-diphosphate concentrations in 10 mouse It appearsthat, in both brain and muscle,glucose-1,6-di-P tissueswere found to range from 70 to 80 pmolesper kg in can by synthesized by P-glucomutasefrom fructose-l, 6-di-P brain and red blood cells to 7 to 10 pmoles per kg in testes and glucose-6-P. There wasno discernible correlation between took place when Paladini et al [3] observedglucoselevels of glucose 1,6-diphosphate and levels of glucose 6- 1,6-di-P formation from glucose-l-PandATP in muscleextracts. Phosphate,uridine diphosphoglucoseor phosphoglucomutase, Very recently, Alpers [4] has found that glucose-l,6-di-P can or Michaelis constants of phosphoglucomutasefor its coen- be synthesized by P-glucomutasefrom 1,3-diphosphozyme. Evidence is presented to show that glucose 1,6-di-P in concerningthe reaction of fructose phosphateswith P-glucomumuscle can be synthesized by P-glucomutase itself from tase[5]. The rate of synthesisis only about 0.015% of the mutase rate for EXPERIMENTAL
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