Abstract
The gluconic acid batch fermentation was conducted using mutant Aspergillus niger NCIM 530 strain under submerged condition in 50 L semiautomatic stirred-tank fermenter. Certain cost-effective source as golden syrup was effectively utilized instead of glucose for successful industrial fermentation process. The significant level of gluconic acid (85.2 gL-1) production was observed with maximum 86.97% glucose conversion over 44 hours. This process provides great advantages over traditional submerged fermentation strategies and substrates, as showed by effective production of gluconic acid by utilizing novel substrate as a golden syrup. To reduce analysis time with better accuracy, an effort has been made to use a method for evaluation of parameters like conversion of substrate and production of gluconic acid during the fermentation by using High Performance Thin Layer Chromatography (HPTLC).
Highlights
Gluconic Acid (GA) is a multifunctional carbonic acid belonging to bulk chemical, with outstanding properties, including extremely low toxicity, very low corrosiveness, and a capability of forming water soluble complexes with different metal ions
A large quantity of raw material produced in agriculture and industrial process can be utilized for gluconic acid production to minimize the fermentation cost
Fermented broth samples and reference (Calcium gluconate-Acros Organics Germany; Glucose-Merk) were applied to the plated by means of CAMAG (Switzerland) Linomat V TLC applicator
Summary
Gluconic Acid (GA) is a multifunctional carbonic acid belonging to bulk chemical, with outstanding properties, including extremely low toxicity, very low corrosiveness, and a capability of forming water soluble complexes with different metal ions. A large quantity of raw material produced in agriculture and industrial process can be utilized for gluconic acid production to minimize the fermentation cost. It contains nearly 47% glucose; so it can be effectively utilized for gluconic acid production.
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