Glucocorticoid-induced leucine zipper (GILZ) promotes apoptosis in vitro in neutrophil-like cells.

Abstract

Event Abstract Back to Event Glucocorticoid-induced leucine zipper (GILZ) promotes apoptosis in vitro in neutrophil-like cells. Marie-Alix Espinasse1*, Pascale Piednoir2, Guillaume Dufour2, Philippe Montravers2, Sylvie Chollet-Martin1, 2, Marc Pallardy1 and Armelle Biola-Vidamment1 1 INSERM UMRS 996, Université Paris-Sud, France 2 APHP-Bichat, Intensive Care Unit, France Glucocorticoid-induced leucine zipper (GILZ) is an ubiquitous potent anti-inflammatory protein whose expression is induced by glucocorticoids, TGF- or IL-10 in haematopoietic cells. We and others have evidenced that GILZ regulates inflammation -involved signal transduction pathways, such as AP-1, NF-kappaB or FOXO. Moreover, we recently detected GILZ expression in blood neutrophils of patients suffering from acute respiratory distress syndrome (ARDS). The objective of this study was to evaluate the role of GILZ in human neutrophils apoptosis. For this purpose, we used neutrophil-like PLB-985 differentiated cells. We generated GILZ overexpressing cells stably transfected with the human gilz gene. These cells were then differentiated in neutrophil-like cells using all trans-retinoic acid (ATRA) and dimethylformamid (DMF). We measured CD11b expression, oxidative burst, phagocytosis capacity and verified the presence of multi-lobed nuclei. Differentiated PLB-985 cells were then characterized for their spontaneous and activation-induced apoptosis using PMA through SubG1 analysis and AnnexinV/7AAD staining. We found that GILZ overexpression led to an exacerbated apoptosis, characterized by caspase 3, 9 and 8 activation and loss of mitochondrial potential. These results suggested that GILZ-induced apoptosis involved the mitochondrial pathway. Interestingly, apoptosis of PLB-985 GILZ clones was associated with a downregulation of Mcl-1, whereas BID or BIM were not involved. This model suggests that in neutrophils, GILZ could participate in the control of apoptosis, essential to the resolution of inflammation in vivo. Further investigations of GILZ functions in neutrophils are ongoing both in vitro and ex vivo during ARDS. Keywords: Neutrophils, Apoptosis Regulatory Proteins, GILZ, Inflammation, Mcl-1 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Espinasse M, Piednoir P, Dufour G, Montravers P, Chollet-Martin S, Pallardy M and Biola-Vidamment A (2013). Glucocorticoid-induced leucine zipper (GILZ) promotes apoptosis in vitro in neutrophil-like cells.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00730 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Marie-Alix Espinasse, INSERM UMRS 996, Université Paris-Sud, Chatenay-Malabry, France, marie-alix.espinasse@u-psud.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Marie-Alix Espinasse Pascale Piednoir Guillaume Dufour Philippe Montravers Sylvie Chollet-Martin Marc Pallardy Armelle Biola-Vidamment Google Marie-Alix Espinasse Pascale Piednoir Guillaume Dufour Philippe Montravers Sylvie Chollet-Martin Marc Pallardy Armelle Biola-Vidamment Google Scholar Marie-Alix Espinasse Pascale Piednoir Guillaume Dufour Philippe Montravers Sylvie Chollet-Martin Marc Pallardy Armelle Biola-Vidamment PubMed Marie-Alix Espinasse Pascale Piednoir Guillaume Dufour Philippe Montravers Sylvie Chollet-Martin Marc Pallardy Armelle Biola-Vidamment Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.