Abstract
Abstract The specific interaction of glucocorticoids with fetal and adult rabbit tissues was studied in vitro. Specific cytoplasmic binding sites for [3H]dexamethasone were demonstrated in liver, thymus, kidney, lung, muscle, heart, small intestine, skin, brain, and placenta of the rabbit fetus as well as in liver, thymus, spleen, kidney, small intestine, and lung of the adult rabbit. In the rabbit fetus at 28 to 30 days of gestation the concentration of specific binding sites for [3H]dexamethasone was higher (1.62 to 1.83 pmoles per mg of DNA) in placenta, kidney, and lung, somewhat lower (0.69 to 1.25 pmoles per mg of DNA) in skin, muscle, small intestine, and heart, and much lower (0.35 to 0.49 pmole per mg of DNA) in liver, thymus, and brain. Sucrose density gradient analysis of tissue extracts showed the presence of one or two [3H]dexamethasone-macromolecular complexes sedimenting at 7 to 8 S and 4 S. The 7 to 8 S complex was present in all tissues, but the 4 S complex was detected only in liver, kidney, small intestine, and spleen. These results demonstrate the presence of similar [3H]-dexamethasone-binding components (receptors) in a variety of fetal and adult rabbit tissues. It is suggested that in addition to the liver and thymus, which are usually regarded as the target tissues for glucocorticoids, several other rabbit tissues may also be targets for these hormones.
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