Abstract
SPECIFIC AIM>5-lipoxygenase (5LOX) is the key enzyme in the synthesis of inflammatory leukotrienes (LTs) from arachidonic acid. We had previously found that hyperglucocorticoidemia or dexamethasone treatment increase 5LOX expression in the rat brain in vivo. The aim of this study was to characterize in detail the stimulatory action of dexamethasone on 5LOX expression in neural cultures in vitro.PRINCIPAL FINDINGS1. Dexamethasone increases 5LOX mRNA and protein content in primary cultures of rat cerebellar granule neurons (CGN) in a concentration- and time-dependent mannerExposure of CGN to dexamethasone increased the content of both 5LOX mRNA (assayed by quantitative RT-PCR) and 5LOX protein (Western blotting). These increases were concentration dependent and became statistically significant with 100 nM dexamethasone. Time course studies revealed that dexamethasone treatment increased the content of 5LOX mRNA and protein as early as 3 h and that these increases persisted for at least 24 h of dexamethasone...
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