Glucocorticoid-induced proteins in bovine endothelial cells

Abstract

High circulating levels of corticosteroids are associated with elevated blood pressure and an increased incidence of vascular damage. We have used high resolution two-dimensional gel electrophoresis and autoradiography to analyse direct steroid effects on the newly synthesized proteins in bovine aortic endothelial cells. At medium concentrations of 10 −7 M, corticosteroids but not sex steroids increased the synthesis of two endothelial proteins: el (M.W. $ ̃ 43K, pI $ ̃ 6.3) and e2 (M.W. $ ̃ 28K, pI $ ̃ 5.9). The responses were consistent for different endothelial cell cultures, different gel runs, and for natural and synthetic glucocorticoids, including the “pure” synthetic glucocorticoid, RU 26988. Mineralocorticoids (aldosterone, deoxycorticosterone) at 10 −7 M were less potent in their effect on the synthesis of e2, and had no discernible effect on the synthesis of el. These specific effects on cellular protein synthesis demonstrate that glueocorticoids can act directly on endothelial cells. These direct actions may thus mediate altered endothelial cell function in clinical and experimental cardiovascular conditions characterized by corticosteroid excess.