Abstract
Background/Purpose: This study was designed to explore the efficacy of a synthetic analogue of glucagonlike peptide-2 (GLP-2α) in enhancing mucosal mass and absorptive function in a rat model of intestinal ischemia-reperfusion (I-R) injury. Methods: Each of 20 Sprague-Dawley rats underwent placement of a jugular venous catheter connected to a subcutaneous osmotic pump designed to deliver its contents over 3 days. Rats were divided into 4 groups (n = 5 per group): (1) normal intestine/saline infusion; (2) 30-minute superior mesenteric artery occlusion/saline infusion, (3) normal intestine/GLP-2α infusion, and (4) 30-minute superior mesenteric artery occlusion/GLP-2α infusion. Subsequently, mean mucosal 14C-galactose and 14C-glycine absorption and DNA content were determined for each group. Results: In saline-treated rats, 30 minutes of mesenteric ischemia decreased mean mucosal galactose absorption by 29% (P <.05), glycine absorption by 22% (P =.12), and DNA content by 28% (P <.01) when compared with rats with uninjured intestine. In rats subjected to 30 minutes of intestinal ischemia, GLP-2α significantly improved galactose absorption by 46% (P <.05), glycine absorption by 84% (P <.01), and DNA content by 63% (P <.01) when compared with saline-treated control rats. In rats with mesenteric I-R injury treated with GLP-2α, galactose absorption was returned to normal. Glycine absorption and DNA content were increased significantly by 44% (P <.01) and 18% (P <.05), respectively, beyond the baseline for normal intestine. Conclusions: Thirty minutes of intestinal ischemia followed by immediate reperfusion significantly decreased mucosal mass and absorptive function, validating this rat model of I-R injury. After mesenteric I-R, GLP-2α significantly increased mucosal DNA content and absorption of 14C-galactose and 14C-glycine when compared with untreated control rats. After I-R injury, GLP-2α restored mucosal mass and absorptive function to normal or above-normal levels. J Pediatr Surg 35:1537-1539. Copyright © 2000 by W.B. Saunders Company.
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