Glucagon and plasma lipoprotein lipase.

Abstract

SummarySubcutaneous administration of 1.0 mg glucagon to 15 fasted normal people caused significant increase of plasma lipoprotein lipase-like activity in 1 hr. The activity is termed lipase-like since coconut oil emulsion rather than isolated chylomicrons was used as substrate. A linear relationship between lipolysis and time of incubation obtained indicating zero order kinetics. pH optimum of enzyme activity was 8.4. There was no correlation between depression of plasma triglycerides and increase -of plasma lipoprotein lipase-like activity following glucagon administration. This was thought to be due to other effects of glucagon administration which alter plasma lipid levels. Incubation of whole blood with glucagon caused an increase of plasma lipoprotein lipase-like activity and a depression of plasma triglycerides that were significantly correlated. The in vitro action of glucagon when incubated with whole blood suggests that blood cell(s) may be a source of glucagon-induced plasma lipoprotein lipase ...