Abstract
Adenyl cyclase has been shown to be specifically localized in the plasma membrane of rat liver. The activity of this enzyme is stimulated by glucagon and epinephrine in isolated plasma membrane systems. The stimulation by epinephrine has a lag period of about 10 minutes. Epinephrine shows selective binding to isolated plasma membranes. The results indicate that epinephrine binds to a receptor protein rather than interacting directly with the enzyme adenyl cyclase. Calcium ions also stimulate adenyl cyclase in isolated plasma membranes but the effect shows appreciable variability.
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