Abstract
Abstract The attractant (orange light) or repellent (white light) signal is transmitted from SRI (Sensory Rhodopsin I) via protein‐protein interaction with its transducer HtrI (Halobacterial Transducer for Sensory Rhodopsin I) which in turn controls a cytoplasmic phospho‐transfer pathway that modulates flagella motor switching in Halobacterium salinarum. Some mutations in both SRI and HtrI showed an unusual mutant phenotype called inverted signaling, in which the cell produces a repellent response to normally attractant light. Twelve mutations at the Glutamate 56 (E56) position in the second transmembrane helix of HtrI were introduced by site‐specific random mutagenesis. Almost all E56 mutants showed orange‐light inverted responses in pH and temperature‐dependent manners except E56D and E56Y. Except for these two mutants, all mutants accelerated the S373 decay compared to wild‐type at 18°C. This supported that there is an interaction between SRI and the second transmembrane of HtrI. Also a structural mode...
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