Abstract
Acute kidney injury (AKI), a global health problem that affects approximately 13 million people a year, represents one of the primary complications of renal injury due to ischemia-reperfusion. Ischemia-reperfusion occurs in many clinical scenarios, including renal transplantation, vascular surgery, and shock. Increased oxidant production and/or the decrease of antioxidant enzymes, contribute to the tissue damage that occurs during ischemia- reperfusion-reoxygenation injury (IRRI). Glucagon-Like perpide-1 receptor agonist (GLP-1r) activation has renoprotective effects in diabetes through its anti-inflammatory action. However, it is not known whether GLP-1r activation improves the renal antioxidant response after ischemia-reperfusion. Therefore, the objective of this study was to assess whether the renal protective effects of GLP-1r activation on renal IRRI reduces the oxidative stress and the hypoxia. We assessed the gene expression of HIF-1 a, NOX4, HMOX1, SOD1 and SOD2 in the kidney before and after 30 minutes of ischemia and 24 hours of reperfusion in the following group of rats: (1) untreated Long-Evans Tokushima Otsuka rats (n=7), (2) untreated Otsuka Long-Evans Tokushima Fatty rats (OLETF) (n=8), and (3) OLETF+GLP-1r agonist (EXE; 10 μg exenatide/kg/day (n=6). The induction of ischemia-reperfusion increased HIF-1α mRNA expression in untreated OLETF approximately 4-fold compared to LETO group, and OLETF + GLP-1r decreased it 100% compared to LETO group and 6-fold compared to untreated OLETF. The preliminary data suggest that GLP-1r is protective against renal IRRI due to the decrease in the gene expression of HIF-1α during ischemia-reperfusion and during chronic treatment in insulin resistant rats Support or Funding Information Special thank to the APS fellowship “UGRSRF”. To UC Merced and Research Host PhD. Rudy M. Ortiz. To the University of Sonora in México for the guidance and financing in airplane tickets to can travel to California. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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