Abstract
Proteins that luminesce when they bind calcium are useful tools for studying calcium signaling and homeostasis. Torrecilla et al . generated aequorin-expressing cyanobacteria to determine whether these bacteria used calcium as a signal in response to temperature shock. First, the authors characterized the calcium homeostasis mechanism and found a biphasic response to increases in extracellular calcium with the slower decay phase dependent on both the reduction of [Ca 2+ ] i by calcium binding proteins and calcium transporters. Calcium transients were generated in response to both heat and cold shock. The mechanisms of increased [Ca 2+ ] i were different: Increased [Ca 2+ ] i produced by heat shock arises from calcium released from both intracellular stores and by influx from the extracellular space, and increased [Ca 2+ ] i produced by cold shock arises from predominantly extracellular sources. Torrecilla, I., Leganés, F., Bonilla, I., and Fernández-Piñas, F. (2000) Use of recombinant aequorin to study calcium homeostasis and monitor calcium transients in response to heat and cold shock in cyanobacteria. Plant Physiol. 123 : 161-175. [Abstract] [Full Text]
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