Global transcriptomic analysis reveals Lnc-ADAMTS9 exerting an essential role in myogenesis through modulating the ERK signaling pathway

Liqi Wang,Xin Zhang,Linjuan He,Kai Qiu,Jingdong Yin,Ning Jiao,Ting He,Yubo Wang

doi:10.1186/s40104-020-00524-4

Liqi Wang, Xin Zhang + Show 6 more

Open Access

https://doi.org/10.1186/s40104-020-00524-4

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Abstract

BackgroundLong non-coding RNAs (lncRNAs) are emerging key regulators involved in a variety of biological processes such as cell differentiation and development. The balance between myogenesis and adipogenesis is crucial for skeletal muscle homeostasis in humans and meat quality in farm animals. The present study aimed to reveal the global transcriptomic profiles of adipogenic (Adi-) and myogenic (Myo-) precursors derived from porcine skeletal muscle and identify lncRNAs involved in the modulation of myogenesis homeostasis in porcine skeletal muscle.ResultsIn this study, a total of 655 novel individual lncRNAs including differentially expressed 24 lncRNAs, and 755 differentially expressed mRNAs were identified (fold change ≥2 or ≤ 0.5 and adjusted P < 0.05). Integrated results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis accompanied by the variation of intracellular Ca2+ concentration highlighted Lnc-ADAMTS9 involved in the modulation of myogenesis homeostasis in porcine skeletal muscle. Although Lnc-ADAMTS9 knock-down did not alter the mRNA expression of ADAMTS9, we demonstrated that Lnc-ADAMTS9 can promote myogenic proliferation and myogenic differentiation of myogenic precursors through inhibiting the ERK/MAPK signaling pathway.ConclusionWe deciphered a comprehensive catalog of mRNAs and lncRNAs that might be involved in the regulation of myogenesis and adipogenesis homeostasis in the skeletal muscle of pigs. The Lnc-ADAMTS9 exerts an essential role in myogenesis through the ERK signaling pathway.

Highlights

The skeletal muscle makes up 20% – 50% of the total body mass in adult mammals and plays a pivotal role in regulating body metabolism and homeostasis [1]
Characterization of adipogenic and myogenic precursors Upon adipogenic induction, adipogenic precursors differentiated into mature adipocytes, demonstrated by accumulating lipid droplets shown by Oil Red O staining (Fig. 1a, b)
We investigated the roles of Long non-coding RNAs (lncRNAs) in the modulation of myogenesis and adipogenesis homeostasis in skeletal muscle by comparing global transcriptomic landmark between myogenic and adipogenic precursors derived from the skeletal muscle of pigs

Summary

Introduction

The skeletal muscle makes up 20% – 50% of the total body mass in adult mammals and plays a pivotal role in regulating body metabolism and homeostasis [1]. Myogenesis is a crucial step for skeletal muscle development, regeneration, while excessive adipogenesis in skeletal muscle would suppress myogenesis and lead to myofibers infiltrated by lipids, and resultantly damages skeletal muscle contractive function and metabolism homeostasis [5]. The imbalance between myogenesis and adipogenesis in the skeletal muscle leads to various diseases including type-II diabetes, muscular atrophy, sarcopenia, and muscular dystrophy [6]. Long non-coding RNAs (lncRNAs) are emerging key regulators involved in a variety of biological processes such as cell differentiation and development. The balance between myogenesis and adipogenesis is crucial for skeletal muscle homeostasis in humans and meat quality in farm animals. The present study aimed to reveal the global transcriptomic profiles of adipogenic (Adi-) and myogenic (Myo-) precursors derived from porcine skeletal muscle and identify lncRNAs involved in the modulation of myogenesis homeostasis in porcine skeletal muscle

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