Abstract

Routine seed health testing is carried out in most countries for seed certification and plant quarantine. However, the majority of seed health tests used throughout the world have never been subject to rigorous validation. A fully validated test provides for analytical sensitivity, reproducibility and repeatability. Discrepancies between testing methods can occur, leading to costly phytosanitary disputes or liability claims. These issues can be avoided by working toward a system of universally accepted, standardized testing methods on a global level. To ensure that seed health tests are standardized and give reliable and reproducible results in accordance with the given specifications of the test methods, methods should go through a peer review system and/or collaborative study among laboratories. Three primary organizations publish standardized seed health tests: the International Seed Testing Association (ISTA), the International Seed Health Initiative (ISHI), and the U.S. National Seed Health System (NSHS). In 1957, the ISTA Plant Disease Committee (PDC) established a comparative seed health testing program to standardize techniques for detection of seed-borne pathogens. In 1993, the Seed Health Committee (SHC, formerly the PDC) began development of published guidelines for comparative testing. All ISTA validated methods are published in the International Rules for Seed Testing. Additionally, some ISHI-Veg methods have been accepted as ISTA Rules and as Standards by the NSHS. The procedures followed by ISTA, ISHI and the NSHS to achieve global standards in seed health testing are discussed.

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