Abstract
Periodic starvation of animals induces large shifts in metabolism but may also influence many other cellular systems and can lead to adaption to prolonged starvation conditions. To date, there is limited understanding of how starvation affects gene expression, particularly at the protein level. Here, we have used mass-spectrometry-based quantitative proteomics to identify global changes in the Caenorhabditis elegans proteome due to acute starvation of young adult animals. Measuring changes in the abundance of over 5,000 proteins, we show that acute starvation rapidly alters the levels of hundreds of proteins, many involved in central metabolic pathways, highlighting key regulatory responses. Surprisingly, we also detect changes in the abundance of chromatin-associated proteins, including specific linker histones, histone variants, and histone posttranslational modifications associated with the epigenetic control of gene expression. To maximize community access to these data, they are presented in an online searchable database, the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd/).
Highlights
Periodic starvation of animals induces large shifts in metabolism but may influence many other cellular systems and can lead to adaption to prolonged starvation conditions
Response of the C. elegans Proteome to Acute Starvation Stress—To provide a comprehensive overview of the response of the C. elegans proteome to starvation, we performed a time course of food deprivation with SILAC labeled nematodes
Using a combination of in-depth, quantitative proteome analyses and genetic disruption of protein function, we have characterized the global response of the C. elegans proteome to acute starvation
Summary
Global proteomics analysis of the response to starvation in C. elegans Larance, Mark; Pourkarimi, Ehsan; Wang, Bin; Brenes Murillo, Alejandro; Kent, Robert; Lamond, Angus I. Conserved genetic pathways have been identified that link nutrient availability to metabolic remodeling, stress resistance pathways, and enhanced life span Many of these pathways overlap and include TOR, AMPK, autophagy, and insulin/ IGF-1 signaling. A study on rodents focused on ϳ200 selected proteins involved in metabolism and insulin signaling that were extracted from mouse livers This analysis of livers derived from mice that were fed on either a normal or high fat diet and both compared in response to starvation revealed differences in protein levels that were dependent on the genetic background of the mouse strains analyzed [16]. We detect specific histone variants and posttranslational modifications that are regulated in response to starvation
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