Global Gene Expression of Kosteletzkya virginica Seedlings Responding to Salt Stress.

Xiaoli Tang,Hongyan Wang,Chuyang Shao,Hongbo Shao,Keqiang Wu

doi:10.1371/journal.pone.0124421

Abstract

Soil salinization is becoming a serious threat to crop yield all over the world. Nowadays, acquainting the specific molecular mechanisms underlying various abiotic stresses especially to salt stress should be of great importance. While the development of the high-throughout sequencing technology promoted the progress powerfully. The intricate perception, transduction and regulation mechanisms underlying salt stress are being illustrated more and more clearly. As a perennial halophytic plant, Kosteletzkya virginica is able to help us to understand the mechanisms more directly and effectively. We carried out the whole transcriptome analysis on young seedlings with or without salt treatment through high-throughout sequencing technology. The results revealed that the numbers of different expressed transcripts between control and different treatments are 4145 and 9134, respectively. The ORF prediction suggested that there were 94308 ORF out of the 103489 (91.10%) total transcripts. We also carried out further differential expression analysis through gene ontology (GO) classification, cluster of orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In a word, our transcriptome study on Kosteletzkya virginica would provide direct and effective references for researches on molecular mechanisms of salt-tolerance, extending our view of salt tolerance in plant further. Above all, the related report in this paper is the first about Kosteletzkya virginica.

Highlights

Soil salinity is becoming a critical challenge to agriculture worldwide rather than an agonizing problem merely in arid and semi-arid regions
The aim of our research was to learn more about the salt tolerance mechanisms and sought out the functional genes participated in, so we employed edgeR to identify differentially expressed mRNAs based on their relative abundance which was reflected by counting individual read between the two libraries [34,35]
To get a comprehensive view of Kosteletzkya virginica salt tolerance, two-week old seedlings of Kosteletzkya virginica were poured with 1/2 Hoagland and 1/2 Hoagland with the concentration of 300 mM NaCl respectively to study the high salinity responses

Summary

Introduction

Soil salinity is becoming a critical challenge to agriculture worldwide rather than an agonizing problem merely in arid and semi-arid regions. FAO has reported that as much as 6% of the total world land is subjected to salinity [1]. The salt stress caused considerable crop yield losses and restricted the advances in agriculture [2]. The condition is compounded by the growing agricultural need to provide enough nutrition for the world population, which is increasing steadily [3]. Confronted with the serious challenge, scientists are sparing no efforts to work out and the researches on plant science have assisted us a lot to PLOS ONE | DOI:10.1371/journal.pone.0124421. Confronted with the serious challenge, scientists are sparing no efforts to work out and the researches on plant science have assisted us a lot to PLOS ONE | DOI:10.1371/journal.pone.0124421 April 22, 2015

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