Abstract
Gene expression profiling of the response of Schizosaccharomyces pombe cells to loss of the catalytic subunit of telomerase (trt1+) identified two waves of altered gene expression and a continued up-regulation of Core Environmental stress Response (CESR) genes.
Highlights
Schizosaccharomyces pombe cells lacking the catalytic subunit of telomerase lose telomeric DNA and enter crisis, but rare survivors arise with either circular or linear chromosomes
In the absence of a mechanism to compensate for this 'end-replication problem', progressive telomere shortening leads to replicative senescence, which in yeast is characterized by chromosome instability and low cell viability [4,5]
We report that survivors with circular chromosomes maintain an extended stress response not observed in survivors with linear chromosomes
Summary
Schizosaccharomyces pombe cells lacking the catalytic subunit of telomerase (encoded by trt1+) lose telomeric DNA and enter crisis, but rare survivors arise with either circular or linear chromosomes. Http://genomebiology.com/2004/6/1/R1 the duplex repeated region varies, from 20 base-pairs (bp) in hypotrichous ciliated protozoa to around 300 bp in yeast and several kilobases (kb) in mammalian cells. These DNA repeats recruit telomeric proteins to form the telosome, a structure that resists nucleolytic degradation and prevents chromosome ends from eliciting recombination and endjoining pathways for repairing double-strand DNA breaks [3]. The catalytic subunit of telomerase is encoded by the gene trt1+ [9]
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