Global correlation analysis for miRNA and protein expression profiles in human peripheral blood mononuclear cells.

Abstract

Micro-RNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression at protein level by protein translation inhibition or mRNA degradation. However, the global correlation patterns between miRNA and protein have not been studied yet. To establish the global correlation patterns in human peripheral blood mononuclear cells (PBMCs), this study conducted multiple types of miRNA-protein correlation analyses in 28 Chinese subjects. Pearson correlation analysis showed a negative but relatively small global correlation in each subject. Among the 371 constructed miRNA-protein pairs (60 unique miRNAs, and 150 unique proteins), 10.5% of pairs have significant correlations (P < 0.05). Some highlighted miRNAs (e.g., hsa-miR-590-3p, hsa-miR-520d-3p) exerted significant regulation on multiple genes. Simultaneously, some genes (e.g., HSP90B1) were targeted by multiple miRNAs. The target genes associated with miRNAs tend to enrich in some important GO terms: biological processes (e.g., gene expression, protein binding and RNA binding), and molecular functions (protein binding: GO:0005515; RNA binding: GO:0003723). The results provided a global view of the miRNA-protein expression correlation profile in human PBMCs, which would facilitate in-depth investigation of biological functions of key miRNAs/proteins and better understanding of the pathogenesis underlying PBMC related diseases.