Abstract
A microarray-based analysis of mRNA half-lives in two species of Sulfolobus, an hyperthermophilic archaeon, shows that their mRNA half-life distribution is similar to that of much faster growing bacteria
Highlights
Transcript half-lives differ between organisms, and between groups of genes within the same organism
The analysis was focused on the S. solfataricus transcriptome, but S. acidocaldarius was surveyed for general trends, providing an independent biological replicate
Previous genome-wide mRNA half-life analyses have indicated that median half-life is roughly proportional to the minimal length of the cell cycle
Summary
Transcript half-lives differ between organisms, and between groups of genes within the same organism The mechanisms underlying these differences are not clear, nor are the biochemical properties that determine the stability of a transcript. To address these issues, genome-wide mRNA decay studies have been conducted in eukaryotes and bacteria. By computational analysis of gene-order conservation in several archaeal genomes, a protein complex orthologous to the eukaryotic exosome was predicted [4] This multisubunit complex consists of RNases, RNA helicases and RNA-binding proteins, in which various RNA classes are degraded in a 3' to 5' fashion. The S. solfataricus exosome was demonstrated to display polyadenylation activity, in addition to degradation of RNA [7]
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