Global analysis of fluorescence decay: applications to some unusual experimental and theoretical studies.

Abstract

AbstractThe methodology for the simultaneous analysis of fluorescence decay curves is shown to create the possibility for some new types of fluorescence decay experiments. These new experiments include the use of the T‐format in anisotropy experiments without the need to match the timing characteristics of photomultiplier tubes. Decay surfaces obtained at multiple timing calibrations can be analyzed simultaneously to resolve widely differing decay rates. Methodologies are described for the discrimination of associative from nonassociative behavior for multiexponential anisotropy decay. A systems theory approach to the analysis of excited state reactions is described.