Abstract
Endocytosis of receptors at the plasma membrane is controlled by a complex machinery that includes clathrin, adaptors, and actin regulators. Many of these proteins are conserved in yeast yet lack observable mutant phenotypes, suggesting yeast endocytosis may be subject to different regulatory mechanisms. We have systematically defined genes required for internalization using a quantitative genome‐wide screen that monitors localization of the yeast VAMP/Synaptobrevin homolog Snc1. Genetic interaction mapping placed these genes into functional modules containing known and novel endocytic regulators and uncharacterized factors. We demonstrate a cargo‐specific requirement for clathrin and the yeast AP180 clathrin adaptor proteins. In addition, we identify a novel conserved component of the endocytic machinery that is recruited to cortical actin patches prior to actin polymerization and is required for normal coat dynamics and actin assembly. Our findings highlight the conserved machinery and reveal novel mechanisms that underlie endocytic recycling.
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