Glial extracellular matrix modulates γ-glutamyl transpeptidase activity in cultured bovine brain capillary and bovine aortic endothelial cells

Abstract

Glial extracellular matrix (ECM) elevated γ-glutamyl transpeptidase (γ-GTP) activity in cultured bovine brain capillary and aortic endothelial cells (BBCEC, BAEC). In particular, the ECM of glial cells cultured with the conditioned medium of BAEC (BAEC CM) dramatically elevated γ-GTP activity in BBCEC and BAEC. The ECM of glial cells cultured with BBCEC CM also had a marked effect. The ECM of 3T3 cells cultured with BAEC CM, and the ECM of glial cells cultured with 3T3 CM had no effect. Glial CM had no effect on γ-GTP activity in BBCEC and BAEC. These findings indicate that γ-GTP activity in endothelial cells (EC) is modulated by glial ECM, and that the factor of ECM that affects γ-GTP activity in EC arises from the interaction between glial cells and EC.