Glial expression of Swiss cheese (SWS), the Drosophila orthologue of neuropathy target esterase (NTE), is required for neuronal ensheathment and function.

Sudeshna Dutta,Franziska Rieche,Carsten Duch,Nina Eckl,Doris Kretzschmar

doi:10.1242/dmm.022236

Sudeshna Dutta, Franziska Rieche + Show 3 more

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https://doi.org/10.1242/dmm.022236

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Abstract

ABSTRACTMutations in Drosophila Swiss cheese (SWS) or its vertebrate orthologue neuropathy target esterase (NTE), respectively, cause progressive neuronal degeneration in Drosophila and mice and a complex syndrome in humans that includes mental retardation, spastic paraplegia and blindness. SWS and NTE are widely expressed in neurons but can also be found in glia; however, their function in glia has, until now, remained unknown. We have used a knockdown approach to specifically address SWS function in glia and to probe for resulting neuronal dysfunctions. This revealed that loss of SWS in pseudocartridge glia causes the formation of multi-layered glial whorls in the lamina cortex, the first optic neuropil. This phenotype was rescued by the expression of SWS or NTE, suggesting that the glial function is conserved in the vertebrate protein. SWS was also found to be required for the glial wrapping of neurons by ensheathing glia, and its loss in glia caused axonal damage. We also detected severe locomotion deficits in glial sws-knockdown flies, which occurred as early as 2 days after eclosion and increased further with age. Utilizing the giant fibre system to test for underlying functional neuronal defects showed that the response latency to a stimulus was unchanged in knockdown flies compared to controls, but the reliability with which the neurons responded to increasing frequencies was reduced. This shows that the loss of SWS in glia impairs neuronal function, strongly suggesting that the loss of glial SWS plays an important role in the phenotypes observed in the sws mutant. It is therefore likely that changes in glia also contribute to the pathology observed in humans that carry mutations in NTE.

Highlights

INTRODUCTION swiss cheese mutant flies show degeneration of the adult nervous system that is detectable around day 5 of adulthood by the formation of spongiform lesions in the brain (Kretzschmar et al, 1997)
neuropathy target esterase (NTE) was first linked to neuronal degeneration in the 1930s, when thousands of people were paralyzed after consuming a beverage (Jamaica Ginger) that contained the organophosphorus compound tri-ortho-cresyl phosphate (TOCP)
We detected glial changes in the neuropils, but, whereas the subperineurial/pseudocartridge glia seem to react to the loss of SWS by forming excessive glial processes, the glial cells associated with the neuropil showed stunted glial processes

Summary

Introduction

INTRODUCTION swiss cheese (sws) mutant flies show degeneration of the adult nervous system that is detectable around day 5 of adulthood by the formation of spongiform lesions in the brain (Kretzschmar et al, 1997). SWS is an evolutionarily conserved protein, which, in mammals, is called neuropathy target esterase (NTE) or patatin-like phospholipase domain containing 6 (PNPLA6) (Lush et al, 1998). Organophosphate treatment induces degeneration and locomotion deficits in flies (Wentzell et al, 2014) Both NTE and SWS have several cyclic-nucleotide-binding sites (Lush et al, 1998; Moser et al, 2000), and a domain that can bind to and inhibit the PKA-C3 catalytic subunit of Protein kinase A (Bettencourt da Cruz et al, 2008). The idea that SWS is autonomously required in glia was suggested by experiments expressing SWS in neurons in sws mutants, which

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