Abstract
Gliadin protein components from Aegilops longissima were separated by two-dimensional electrophoresis. No equivalents for α-gliadin were noted. Addition and substitution lines of Ae. longissima in Triticum aestivum 'Chinese Spring' allowed the identification of homoeologous Gli-1 and Gli-2 loci in Ae. longissima chromosomes 1S1 and 6S1. The chromosomal constitution of the alien addition lines was ascertained by C-banding. In addition, C-banding analysis revealed that the Ae. longissima addition set was incomplete as only six distinct addition lines were identified. No evidence for structural modifications between the alien chromosomes in the lines and their Ae. longissima counterparts was found.Key words: gliadins, C-banding, gene location, Aegilops longissima, wheat.
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