Abstract
BackgroundThe yeast Saccharomyces boulardii is used worldwide as a probiotic to alleviate the effects of several gastrointestinal diseases and control antibiotics-associated diarrhea. While many studies report the probiotic effects of S. boulardii, no genome information for this yeast is currently available in the public domain.ResultsWe report the 11.4 Mbp draft genome of this probiotic yeast. The draft genome was obtained by assembling Roche 454 FLX + shotgun data into 194 contigs with an N50 of 251 Kbp. We compare our draft genome with all other Saccharomyces cerevisiae genomes.ConclusionsOur analysis confirms the close similarity of S. boulardii to S. cerevisiae strains and provides a framework to understand the probiotic effects of this yeast, which exhibits unique physiological and metabolic properties.
Highlights
The yeast Saccharomyces boulardii is used worldwide as a probiotic to alleviate the effects of several gastrointestinal diseases and control antibiotics-associated diarrhea
Saccharomyces boulardii (Sb), a yeast strain, is widely used as a probiotic to treat a variety of conditions [4] including antibiotics-associated diarrhea and recurrent Clostridium difficile infection
Genome characteristics Next-generation sequencing of Sb Econorm - Dr Reddy’s Laboratories (EDRL) on the Roche 454 GS-FLX Titanium platform resulted in a total of 733,390 shotgun reads of length 40–1773 bp
Summary
The yeast Saccharomyces boulardii is used worldwide as a probiotic to alleviate the effects of several gastrointestinal diseases and control antibiotics-associated diarrhea. While many studies report the probiotic effects of S. boulardii, no genome information for this yeast is currently available in the public domain. Conclusions: Our analysis confirms the close similarity of S. boulardii to S. cerevisiae strains and provides a framework to understand the probiotic effects of this yeast, which exhibits unique physiological and metabolic properties. The most commonly used human probiotics are members of the Lactobacillus and Bifidobacterium species [3]. Besides these bacteria, Saccharomyces boulardii (Sb), a yeast strain, is widely used as a probiotic to treat a variety of conditions [4] including antibiotics-associated diarrhea and recurrent Clostridium difficile infection. Sb is tolerant to various local stresses such as the presence of gastrointestinal (GI) enzymes, bile salts, organic acids etc. and can withstand considerable variations in pH and temperature while transiting through the human GIT [8]
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